Cov raj xa ntshav EDTA yog dab tsi thiab lawv siv li cas?

Hauv kev kuaj mob thiab kev kuaj mob thiab kev kho mob,EDTA cov raj xa ntshav, ua cov khoom siv tseem ceeb rau kev sau ntshav, ua lub luag haujlwm tseem ceeb hauv kev lav qhov tseeb ntawm cov qauv thiab qhov tseeb ntawm kev kuaj. Hauv tsab xov xwm no, peb yuav tshuaj xyuas tag nrho cov "tus saib xyuas tsis pom" no hauv kev kho mob los ntawm cov ntsiab lus ntawm kev txhais, kev faib xim, lub hauv paus ntsiab lus anticoagulation, lub hom phiaj kuaj thiab tus qauv siv.

Yog dab tsiEDTA raj xa ntshav?

EDTA lub raj xa ntshav yog ib hom raj xa ntshav uas muaj Ethylene Diamine Tetraacetic Acid lossis nws cov ntsev, uas feem ntau yog siv rau kev sau cov qauv ntshav thiab kev kho mob anticoagulant. EDTA tuaj yeem thaiv cov tshuaj coagulation cascade los ntawm kev chelating cov calcium ions hauv cov ntshav, kom cov ntshav nyob hauv cov kua dej ntev, thiab muab cov qauv ruaj khov rau kev kuaj ntshav niaj hnub thiab molecular biology. Nws muab cov qauv ruaj khov rau kev kuaj ntshav niaj hnub, molecular biology thiab lwm yam kev kuaj.

Ua ib feem tseem ceeb ntawmcov khoom siv kho mob, EDTA cov raj sau ntshav yuav tsum ua raws li tus qauv hauv tebchaws ntawm "Cov thawv sau ntshav siv ib zaug xwb" (piv txwv li GB / T 19489-2008) kom ntseeg tau tias muaj kev ua haujlwm ntawm kev tsis muaj menyuam, tsis muaj pyrogenic thiab tsis muaj cytotoxicity.

Cov xim sib txawv ntawm EDTA cov raj xa ntshav

Raws li cov qauv thoob ntiaj teb (xws li CLSI H3-A6 cov lus qhia), EDTA cov raj sau ntshav feem ntau yog capted hauv xim av (EDTA-K2/K3) lossis xiav (sodium citrate sib xyaw nrog EDTA) kom paub qhov txawv ntawm kev siv:

Lus Cim: Tej lub hom lag luam yuav muaj coded ua lwm yam xim, xyuas cov lus qhia ua ntej siv.

Txoj kev tiv thaiv kev coagulation ntawm EDTA cov raj xa dej ntshav

EDTA los ntawm nws cov pawg carboxyl molecular (-COOH) thiab calcium ions hauv cov ntshav (Ca²⁺) ua ke los ua ib qho chelate ruaj khov, yog li ntawd inhibiting qhov kev ua haujlwm ntawm plasminogen, thaiv cov txheej txheem coagulation ntawm fibrinogen rau hauv fibrin. Cov tshuaj anticoagulation no muaj cov yam ntxwv hauv qab no:

1. pib ua haujlwm sai: anticoagulation tuaj yeem ua tiav hauv 1-2 feeb tom qab sau ntshav;

2. kev ruaj khov siab: cov qauv tuaj yeem khaws cia rau ntau tshaj 48 teev (lub tub yees tuaj yeem txuas ntxiv mus txog 72 teev);

3. Ntau yam kev siv: tsim nyog rau feem ntau ntawm kev kuaj ntshav, tab sis tsis yog rau kev kuaj ntshav coagulation lossis platelet function (xav tau cov raj sodium citrate).

Cov khoom tseem ceeb ntawm EDTA cov raj xa ntshav

1. kev tshuaj xyuas ntshav niaj hnub: suav cov qe ntshav dawb, cov qe ntshav liab, hemoglobin concentration, thiab lwm yam;

2. kev txheeb xyuas pawg ntshav thiab kev sib phim: ABO pawg ntshav, kev kuaj pom Rh;

3. kev kuaj mob molecular: kev kuaj noob caj noob ces, kev txiav txim siab txog cov kab mob viral (piv txwv li HIV, HBV);

4. glycated hemoglobin (HbA1c): kev soj ntsuam ntshav qab zib mus sij hawm ntev rau tus kab mob ntshav qab zib;

5. kev kuaj ntshav kab mob: Plasmodium, microfilariae nrhiav tau.

Kev siv cov qauv thiab kev ceev faj

1. Cov txheej txheem sau nyiaj:

Tom qab tua kab mob ntawm daim tawv nqaij, ua haujlwm raws li tus qauv ntawm kev sau cov ntshav venous;

Tom qab sau tag, tig lub raj sau ntshav 5-8 zaug kom paub tseeb tias cov tshuaj anticoagulant sib xyaw nrog cov ntshav tag nrho;

Tsis txhob co co hnyav heev (kom tiv thaiv kom tsis txhob muaj ntshav txhaws).

2. Kev khaws cia thiab kev thauj mus los:

Khaws cia rau qhov kub thiab txias hauv chav tsev (15-25 ° C), tsis txhob kub lossis khov;

Muab tso rau ntsug thaum lub sijhawm thauj mus los kom tiv thaiv kom tsis txhob xoob lub hau raj.

3. cov xwm txheej tsis pom zoo:

Cov raj sodium citrate yog qhov yuav tsum tau rau Coagulation IV (PT, APTT, thiab lwm yam);

Kev kuaj mob platelet yuav tsum muaj lub raj mis sodium citrate.

Yuav ua li cas xaiv cov khoom zooEDTA raj xa ntshav?

1. Kev tsim nyog thiab daim ntawv pov thawj: xaiv cov khoom uas tau dhau daim ntawv pov thawj ISO13485 thiab CE. 2;

2. Kev nyab xeeb ntawm cov khoom siv: lub cev raj yuav tsum pob tshab thiab tsis muaj cov khoom seem yas;

3. Kev noj tshuaj kom raug: qhov ntau ntawm cov tshuaj anticoagulant ntxiv yuav tsum ua raws li tus qauv hauv tebchaws (piv txwv li EDTA-K2 concentration ntawm 1.8 ± 0.15mg / mL);

4. Lub koob npe nrov ntawm hom lag luam: Muab qhov tseem ceeb rau cov hom lag luam paub zoo hauv kev siv khoom kho mob kom ntseeg tau tias muaj kev ruaj khov.

Xaus lus

Ua ib tug tswv cuab tseem ceeb ntawmlub cuab yeej sau ntshav, EDTA cov raj xa ntshav muaj qhov cuam tshuam ncaj qha rau qhov tseeb ntawm cov txiaj ntsig ntawm kev xeem hais txog lawv cov khoom anticoagulant. Los ntawm kev siv cov raj xa ntshav sib txawv xim thiab sib xyaw nrog cov txheej txheem sau cov txheej txheem nruj, nws tuaj yeem muab lub hauv paus txhim khu kev qha rau kev kuaj mob. Yav tom ntej, nrog rau kev txhim kho kev kho mob meej, EDTA cov raj xa ntshav yuav ua lub luag haujlwm tseem ceeb hauv kev tshuaj xyuas ntshav, kev txheeb xyuas cov noob caj noob ces thiab lwm yam teb, thiab txuas ntxiv tiv thaiv tib neeg kev noj qab haus huv.