EDTA Blood Collection Tubes yog dab tsi thiab lawv siv li cas?

Hauv kev kuaj mob thiab kev kuaj mob thiab kev kho mob,EDTA cov hlab ntshav, raws li cov khoom siv tseem ceeb rau kev sau ntshav, ua lub luag haujlwm tseem ceeb hauv kev lees paub qhov tseeb ntawm cov qauv thiab qhov tseeb ntawm kev sim. Hauv tsab xov xwm no, peb yuav txheeb xyuas qhov "tus neeg saib xyuas tsis pom" hauv thaj chaw kho mob los ntawm cov ntsiab lus ntawm kev txhais, kev faib xim, cov ntsiab lus ntawm kev tiv thaiv coagulation, lub hom phiaj ntawm kev kuaj thiab siv tus qauv.

Dab tsi yogEDTA ntshav sau tube?

EDTA blood collection tube yog ib hom ntawm lub tshuab nqus tsev sau cov hlab ntsha uas muaj Ethylene Diamine Tetraacetic Acid los yog nws cov ntsev, uas yog siv rau kev sau cov ntshav kuaj thiab kho anticoagulant. EDTA tuaj yeem thaiv cov coagulation cascade cov tshuaj tiv thaiv los ntawm chelating cov calcium ions hauv cov ntshav, thiaj li ua kom cov ntshav nyob hauv cov kua hauv lub sijhawm ntev, thiab muab cov qauv ruaj khov rau kev kuaj ntshav niaj hnub thiab molecular biology. Nws muab cov qauv ruaj khov rau cov ntshav niaj hnub, molecular biology thiab lwm yam kev ntsuam xyuas.

Raws li ib feem tseem ceeb ntawmcov khoom siv kho mob, EDTA cov hlab ntim ntshav yuav tsum tau ua raws li lub teb chaws tus qauv ntawm "Siv ib leeg-siv venous blood samples ntim ntim" (xws li GB / T 19489-2008) kom ntseeg tau tias kev ua haujlwm tsis muaj menyuam, tsis yog pyrogenic thiab tsis-cytotoxicity.

Cov xim sib txawv ntawm cov hlab ntshav EDTA

Raws li cov qauv thoob ntiaj teb (xws li CLSI H3-A6 cov lus qhia), EDTA cov hlab ntshav sau cov hlab ntshav feem ntau yog capped hauv ntshav (EDTA-K2 / K3) lossis xiav (sodium citrate tov nrog EDTA) kom sib txawv ntawm kev siv:

Lus Cim: Qee lub npe yuav raug coded hauv lwm cov xim, xyuas cov lus qhia ua ntej siv.

Anticoagulation mechanism ntawm EDTA cov hlab ntshav sau

EDTA los ntawm nws cov molecular carboxyl pawg (-COOH) thiab calcium ions nyob rau hauv cov ntshav (Ca²⁺) ua ke los tsim ib tug ruaj khov chelate, yog li inhibiting lub activation ntawm plasminogen, thaiv cov txheej txheem coagulation ntawm fibrinogen rau hauv fibrin. Qhov no anticoagulation muaj cov yam ntxwv hauv qab no:

1. kev pib sai sai: anticoagulation tuaj yeem ua tiav hauv 1-2 feeb tom qab sau cov ntshav;

2. kev ruaj ntseg siab: cov qauv tuaj yeem khaws cia rau ntau tshaj 48 teev (cov tub yees tuaj yeem txuas mus rau 72 teev);

3. Kev siv ntau yam: haum rau feem ntau cov kev ntsuam xyuas hematology, tab sis tsis yog rau kev kuaj coagulation lossis platelet muaj nuj nqi (yuav tsum tau siv cov raj sodium citrate).

Cov khoom kuaj tseem ceeb ntawm EDTA cov ntshav sau raj

1. niaj hnub soj ntsuam ntshav: cov ntshav dawb suav, cov qe ntshav liab tsis muaj, hemoglobin concentration, thiab lwm yam;

2. Kev txheeb xyuas pawg ntshav thiab kev sib txuam sib luag: ABO cov ntshav, kuaj Rh factor;

3. Kev kuaj mob molecular: kuaj caj ces, kev txiav txim siab kis kab mob (xws li HIV, HBV);

4. glycated hemoglobin (HbA1c): kev soj ntsuam cov ntshav qabzib mus ntev rau ntshav qab zib mellitus;

5. kuaj ntshav parasite: Plasmodium, microfilariae kuaj.

Kev siv cov cai thiab kev ceev faj

1. Cov txheej txheem sau:

Tom qab tshuaj tua kab mob ntawm daim tawv nqaij, ua haujlwm raws li tus qauv ntawm kev sau cov ntshav venous;

Tam sim ntawd tom qab sau, thim cov hlab ntshav 5-8 zaug kom ntseeg tau tias cov tshuaj tiv thaiv coagulant tau tov nrog cov ntshav;

Tsis txhob ua nruj ua tsiv (kom tiv thaiv hemolysis).

2. Cia thiab thauj:

Khaws ntawm chav tsev kub (15-25 ° C), tsis txhob tshav kub los yog khov;

Muab vertically thaum lub sij hawm thauj mus los los tiv thaiv loosening ntawm lub raj cap.

3. contraindication scenarios:

Sodium citrate tubes yuav tsum tau rau Coagulation IV (PT, APTT, thiab lwm yam);

Platelet muaj nuj nqi kuaj yuav tsum tau sodium citrate raj.

Yuav xaiv qhov zoo li casEDTA ntshav sau tube?

1. Kev tsim nyog thiab ntawv pov thawj: xaiv cov khoom uas tau dhau ISO13485 thiab CE daim ntawv pov thawj. 2;

2. Kev nyab xeeb ntawm cov khoom siv: lub cev lub raj yuav tsum yog pob tshab thiab tsis muaj cov khoom siv yas;

3. Kev noj tshuaj kom raug: tus nqi ntawm cov tshuaj anticoagulant ntxiv yuav tsum ua raws li lub teb chaws tus qauv (xws li EDTA-K2 concentration ntawm 1.8 ± 0.15mg / mL);

4. Hom lub koob npe nrov: Qhov tseem ceeb yog muab rau cov npe nrov hauv kev kho mob cov khoom siv los xyuas kom meej batch stability.

Xaus

Raws li tus tswv cuab tseem ceeb ntawmCov cuab yeej sau ntshav, EDTA cov hlab ntshav sau cov hlab ntsha muaj qhov cuam tshuam ncaj qha rau qhov tseeb ntawm cov txiaj ntsig ntawm qhov ntsuas ntawm lawv cov khoom tiv thaiv coagulant. Los ntawm kev ua qauv siv cov hlab ntshav sib txawv ntawm cov xim sib txawv thiab sib txuas nrog cov txheej txheem sau nruj, nws tuaj yeem muab lub hauv paus txhim khu kev qha rau kev kuaj mob. Nyob rau hauv lub neej yav tom ntej, nrog rau kev txhim kho cov tshuaj precision, EDTA cov hlab ntshav sau cov hlab ntshav yuav ua lub luag haujlwm tseem ceeb hauv kev tshuaj xyuas ntshav, kev sib txuas ntawm noob thiab lwm yam, thiab txuas ntxiv tiv thaiv tib neeg kev noj qab haus huv.